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Isolated T cells were activated with human T activator CD3/CD28 Dynabeads (Life Technologies, 11131D) at a 1:1 cell-to-bead ratio for 24 h before transduction

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These findings suggest that Bdnf expression in the hippocampus is sensitive to fluctuations in leptin availability and leptin signaling, serving as a finely tuned neural component of the adipose-brain axis

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